Sprague-Dawley rats (39 females, 32 males; Charles River, Hollister, CA, USA) began experimental procedures at Post Natal Day (PND) 25 (gavage) or 27 (gelatin) for animals exposed to alcohol, or PND 30, 50, or 120 for neurochemical experiments without alcohol exposure. Rats were housed in polycarbonate tubs on a 12-h light/dark cycle (lights on a 06:00) for one week before this date. Water and rodent chow (Teklad, Harlan, Kent, WA, USA) was available ad libitum except as noted. All work in this manuscript was approved by the Institutional Animal Care and Use Committee of the University of Washington.
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