Evaluating OA Pain Relief Strategies

The pain-related behavior of OA rats after intra-articular injection of various therapeutic agents was evaluated by weight-bearing index (WBI) by a 3D gait analysis system (Kinama Tracer, Japan) and paw-withdrawal threshold (PWT) by the von Frey filament (Ugo Basile, Varese, Italy). Briefly, for WBI detection, rats walked along the runway equipped with mechanical sensors, which can record the ground reaction force for each foot, reacting to the weight supported by the corresponding limb. The values were represented by the following formula: WBI = ipsilateral weight/(ipsilateral weight + contralateral weight) × 100%. PWT was conducted to evaluate the efficacy on pain threshold as described previously (Micheli et al., 2019 (link)). In addition, PWT, reflecting the mechanical allodynia (hypersensitivity), was investigated according to the force exerted by the von Frey filament ranging from 0 to 40 g with a .2-g accuracy. The paw sensitivity threshold was recorded as the minimum force for leading to a strong and immediate withdrawal reflex of the paw.

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Zhu Y., Ye L., Cai X., Li Z., Fan Y, & Yang F. (2022). Icariin-Loaded Hydrogel Regulates Bone Marrow Mesenchymal Stem Cell Chondrogenic Differentiation and Promotes Cartilage Repair in Osteoarthritis. Frontiers in Bioengineering and Biotechnology, 10, 755260.

Publication 2022

von Frey filament

Filament Foot Intra articular injection Mechanical allodynia Pain Rats Reflex Sensitivity Therapeutic

Corresponding Organization : Huadong Hospital

Other organizations : Renji Hospital

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Variable analysis

independent variables

Various therapeutic agents injected intra-articularly

dependent variables

Weight-bearing index (WBI) measured by a 3D gait analysis system
Paw-withdrawal threshold (PWT) measured by the von Frey filament

control variables

Runway equipped with mechanical sensors for WBI detection
Von Frey filament force ranging from 0 to 40 g with 0.2-g accuracy for PWT

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