RNA Extraction and qRT-PCR Analysis

The total RNA was extracted for Chinese cabbage tissues using TRIzol, while yeast RNA was extracted using the M5 EASYspin yeast RNA rapid extraction kit, MF158-01 (Mei5 Biotechnology, Co., Ltd.). For yeast RNA extraction, the cells were grown until the OD₆₀₀ value reached 0.3 at 28 °C and then treated with Hg for 18 h before the total RNA was harvested [4 (link)]. The first-stand cDNA was synthesized using a PrimeScript and RT reagent kit with gDNA Eraser (TAKARA). The SYBR Premix Ex-Taq Kit (TAKARA) was used for quantitative real-time PCR. All experiments were performed with three independent biological replications. The transcript levels were calculated using the 2^ΔΔ-CT method. The TMP values of Chinese cabbage tissues were obtained from the Chinese cabbage database (http://brassicadb.cn/#/, accessed on 18 August 2022) for BrCYP71A15 gene. The primers used for qRT-PCR are presented in Supplementary Table S1.