Plasmid-Based Wnt Pathway Analysis

The following plasmids were used: human DVL2-GFP and FLAG-Axin (Fiedler et al., 2011 (link)); FLAG-DVL2, E499G, K446M, and D460K (Mund et al., 2015 (link)); and SNAP-FZD5 (Koo et al., 2012 (link)). DEP-RFP (red fluorescent protein) was generated by subcloning DEP into DsRed. DEP and FZD5 mutants were generated by standard procedures and verified by sequencing. The following antibodies and resins were used: α-FLAG, α-tubulin, and α-GFP (Sigma); α-actin (Abcam); and α-SNAP (NE Biolabs).

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Gammons M.V., Renko M., Johnson C.M., Rutherford T.J, & Bienz M. (2016). Wnt Signalosome Assembly by DEP Domain Swapping of Dishevelled. Molecular Cell, 64(1), 92-104.

Publication 2016

α-FLAG α-tubulin α-GFP α-actin

Actin Antibodies Human Plasmids Red fluorescent protein Resins α tubulin

Corresponding Organization : MRC Laboratory of Molecular Biology

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Variable analysis

independent variables

Human DVL2-GFP
FLAG-Axin
FLAG-DVL2
E499G
K446M
D460K
SNAP-FZD5
DEP-RFP

dependent variables

Not explicitly mentioned

control variables

α-FLAG
α-tubulin
α-GFP
α-actin
α-SNAP

controls

Positive controls: not specified
Negative controls: not specified

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