Cell Cycle Regulators Profiling

Palbociclib (S1116), abemaciclib (S7158), and ribociclib (S7440) were purchased from Selleck Chemicals. CR-1–31-B and silvestrol were synthesized as described previously (26 (link), 27 (link)). Antibodies against HSP90 (H-114), Cyclin D1 (M20), Cyclin D3 (DCS28), Cyclin A2 (BF683), Cyclin E1 (HE12), Cyclin E2 (A-9), CDK2 (D-12), CDK4 (DCS-35), CDK6 (C-21), p16 (C-20), p21 (H164), and p27 (C-19) were purchased from Santa Cruz Biotechnology; p-RB (S795) and Cyclin D2 (D52F9) were purchased from Cell Signaling Technology; RB (554136) was purchased from BD Pharmingen; and eIF4A1 (ab31217) was purchased from Abcam.

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Kong T., Xue Y., Cencic R., Zhu X., Monast A., Fu Z., Pilon V., Sangwan V., Guiot M.C., Foulkes W.D., Porco JA J.r., Park M., Pelletier J, & Huang S. (2019). eIF4A Inhibitors Suppress Cell-Cycle Feedback Response and Acquired Resistance to CDK4/6 Inhibition in Cancer. Molecular cancer therapeutics, 18(11), 2158-2170.

Publication 2019

Palbociclib abemaciclib ribociclib p-RB (S795) RB (554136)

Abemaciclib Antibodies Cdk2 Cdk6 Cr 31 b Cyclin a2 Cyclin d1 Cyclin d2 Cyclin d3 Cyclin e1 Cyclin e2 Hsp90 Palbociclib Ribociclib Silvestrol

Corresponding Organization : McGill University Health Centre

Other organizations : McGill University, Jewish General Hospital, Boston University

Top 5 similar protocols

Variable analysis

independent variables

Palbociclib (S1116)
Abemaciclib (S7158)
Ribociclib (S7440)
CR-1–31-B
Silvestrol

dependent variables

HSP90
Cyclin D1
Cyclin D3
Cyclin A2
Cyclin E1
Cyclin E2
P-RB (S795)
Cyclin D2
RB (554136)
EIF4A1 (ab31217)

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

Based on most similar protocols

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