Characterization of Synaptic Proteins

Antibodies and chemicals used in this report are as follows: rabbit polyclonal syndecan-2 antibody (syndecan-2G, 1:1000)18 (link); mouse monoclonal PSD-95 (K28/43, MABN68, 1:1000, Millipore)43 (link); mouse monoclonal synaptophysin (S5768, 1:1000, Sigma)44 (link); mouse monoclonal Tubulin (B-5-1-2, 1:5000, Sigma)43 (link); chicken polyclonal GFP (ab13970, 1:5000, Abcam)43 (link); mouse monoclonal GFP (JL-8, 632381, 1:2000, Clontech); rabbit polyclonal Cherry (632496, 1:1000, Clontech); rat monoclonal CD8α (14-0081, 1:1000, eBioscience)45 (link); mouse monoclonal VCP (612183, 1:1000, BD Biosciences)46 (link); mouse monoclonal Myc (9B11, #2276, 1:1000, Cell Signaling Technology)43 (link); mouse monoclonal CASK (K56A, MAB5230, 1:500, Millipore)22 (link); mouse monoclonal Oyster-550-conjugated synaptotagmin (105 311C3, 1:400, Synaptic System); Alexa Fluor-488- and-555-conjugated secondary antibodies (Invitrogen); Sodium chlorate (Sigma); Ethylene glycol tetraacetic acid (EGTA, Sigma); 2-amino-5-phosphonopentanoic acid (AP5; Sigma); KN-92 (Cayman chemical); KN-93 (Sigma); CK-59 (Calbiochem).

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Hu H.T., Umemori H, & Hsueh Y.P. (2016). Postsynaptic SDC2 induces transsynaptic signaling via FGF22 for bidirectional synaptic formation. Scientific Reports, 6, 33592.

Publication 2016

S5768 B-5-1-2 ab13970 Alexa Fluor-488- and-555-conjugated secondary antibodies Sodium chlorate Ethylene glycol tetraacetic acid (EGTA, 2-amino-5-phosphonopentanoic acid (AP5; KN-92 KN-93

2 amino 5 phosphonopentanoic acid Alexa fluor 488 Antibodies Antibody Cayman Cherry Chicken Egta Kn 92 Kn 93 Mouse Oyster Rabbit Sodium chlorate Synaptophysin Synaptotagmin Syndecan Syndecan 2 Tubulin

Corresponding Organization :

Other organizations : Institute of Molecular Biology, Academia Sinica, Boston Children's Hospital, Harvard University

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Variable analysis

independent variables

Rabbit polyclonal syndecan-2 antibody (syndecan-2G, 1:1000)
Mouse monoclonal PSD-95 (K28/43, MABN68, 1:1000, Millipore)
Mouse monoclonal synaptophysin (S5768, 1:1000, Sigma)
Mouse monoclonal Tubulin (B-5-1-2, 1:5000, Sigma)
Chicken polyclonal GFP (ab13970, 1:5000, Abcam)
Mouse monoclonal GFP (JL-8, 632381, 1:2000, Clontech)
Rabbit polyclonal Cherry (632496, 1:1000, Clontech)
Rat monoclonal CD8α (14-0081, 1:1000, eBioscience)
Mouse monoclonal VCP (612183, 1:1000, BD Biosciences)
Mouse monoclonal Myc (9B11, #2276, 1:1000, Cell Signaling Technology)
Mouse monoclonal CASK (K56A, MAB5230, 1:500, Millipore)
Mouse monoclonal Oyster-550-conjugated synaptotagmin (105 311C3, 1:400, Synaptic System)
Alexa Fluor-488- and-555-conjugated secondary antibodies (Invitrogen)
Sodium chlorate (Sigma)
Ethylene glycol tetraacetic acid (EGTA, Sigma)
2-amino-5-phosphonopentanoic acid (AP5; Sigma)
KN-92 (Cayman chemical)
KN-93 (Sigma)
CK-59 (Calbiochem)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

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