Monitoring Ovarian Cancer Progression in Mice

One million of mouse ovarian surface epithelial cancer cells (MOSEC/LUC), which constitutively expressed luciferase, in 100 μl of Hank's balanced salt solution were intraperitoneally injected to each C57BL/6 mouse using a 23-gauge needle (Becton Dickson, Franklin Lakes, NJ, USA). After 2–4 days, mice were injected with luciferin intraperitoneally (100 μl of 0.4 mg/ml luciferin; Goldbio, St. Louis, MO, USA; luck-1) for 10 min, and tumor growth was monitored by luciferase activity detected with the Xenogen IVIS 200 In Vivo Imaging System (Xenogen Corp., Alameda, CA, USA). All mice were sedated with isoflurane before the imaging. Light outputs were quantified using the LivingImage software (Xenogen Corp.), as previously described.^{10 (link)}