Overfed Transgenic Zebrafish EGFP Analysis

Tg (−1.0ins:EGFP) zebrafish were overfed for 10 days with or without PM feeding, as described previously [22 (link)]. The fish were then fasted overnight and anesthetized by placing them in a tank containing 500 ppm of 2-phenoxyethanol (Wako Pure Chemicals, Osaka, Japan). Enhanced green fluorescent protein (EGFP) signaling was captured using a BZ-X710 fluorescence microscope (Keyence, Tokyo, Japan). The EGFP intensity was quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA), as described in our previous study [22 (link)].

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Nakayama H., Shimada Y., Zang L., Terasawa M., Nishiura K., Matsuda K., Toombs C., Langdon C, & Nishimura N. (2018). Novel Anti-Obesity Properties of Palmaria mollis in Zebrafish and Mouse Models. Nutrients, 10(10), 1401.

Publication 2018

2-phenoxyethanol BZ-X710 fluorescence microscope

Enhanced green fluorescent protein Fish Fluorescence microscope Phenoxyethanol Zebrafish

Corresponding Organization : Mie University

Other organizations : Nippon Chemical Industrial (Japan), Oregon State University, Oregon Department of Fish and Wildlife

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Variable analysis

independent variables

Feeding condition (overfed with or without PM feeding)

dependent variables

EGFP intensity

control variables

Tg (-1.0ins:EGFP) zebrafish
Overnight fasting
Anesthetization with 500 ppm of 2-phenoxyethanol
Imaging using a BZ-X710 fluorescence microscope
Quantification of EGFP intensity using ImageJ software

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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