Notch1 Activation in Intracerebral Hemorrhage

As previous study reported [13 (link)], mice were anesthetized intraperitoneally with 4% phenobarbital sodium at a dose of 5 μl/g and implanted with an osmotic minipump (Alzet 1003D, Alza Corporation, CA, USA) in the ipsilateral lateral ventricle after ICH performed. Briefly, the Notch1 activator was prepared as previous described [13 (link)], Notch1-activating antibody (Sigma-Aldrich, St Louis, MO, USA), 10 μg/ml, was dissolved in the artificial cerebrospinal fluid (Sigma-Aldrich, St Louis, MO, USA) at a 1:4 dilution. Then the cannula was placed into the Tleft lateral ventricle (bregma: 0.6 mm posterior, 1.1 mm left lateral and 3.0 mm deep). Each mouse was infused for 3 days with 0.10 µl/h of Notch1-activating antibody by continuous intracerebroventricular infusion. Only artificial cerebrospinal fluid was used for the vehicle of Notch activator in Sham and ICH + Vehicle groups.