DNA synthesis was assessed using an enzyme-linked immunosorbent assay-based and colorimetric BrdU assay (Roche Diagnostics) as previously described [21 (link)]. GIST48 cells transduced with shPLCB4, shYAP1, or shLacZ control were plated into a 96-well plate at density of 3000 cells per well, and DNA synthesis was evaluated at 24, 48, and 72 h. The absorbance of the samples was measured using an ELISA reader (Promega) at 450 nm, with the absorbance at 690 nm as reference.
Li C.F., Liu T.T., Chuang I.C., Chen Y.Y., Fang F.M., Chan T.C., Li W.S, & Huang H.Y. (2017). PLCB4 copy gain and PLCß4 overexpression in primary gastrointestinal stromal tumors: Integrative characterization of a lipid-catabolizing enzyme associated with worse disease-free survival. Oncotarget, 8(12), 19997-20010.
Assay BrduCells Colorimetric Diagnostics Dna synthesis ElisaPromega
Corresponding Organization :
Other organizations :
Southern Taiwan University of Science and Technology, Chi Mei Medical Center, National Health Research Institutes, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University, Kaohsiung Medical University, Kaohsiung Medical University Chung-Ho Memorial Hospital
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