Western Blot Analysis of P2X2 and P2X3 in Diabetic Rats

Expressions of P2X2 and P2X3 in T13-L2 DRGs from diabetic and CON rats were determined using western blotting analysis, as previously described in details^{19 (link), 35 (link)}. The primary antibodies including rabbit anti-P2XRs (1:1000, Abcam, USA), rabbit anti-GAPDH (1:1000, Biotechnology Co., CHN), mouse anti-actin (1:1000; Chemicon, Temecula, CA) and the secondary antibodies including anti-rabbit peroxidase-conjugated secondary antibody (1:2000; Santa Cruz Biotechnology, Santa Cruz, CA), anti-mouse horseradish peroxidase-conjugated secondary antibody (1:4000; Chemicon) were used to probe the target proteins.

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Hu J., Qin X., Song Z.Y., Yang P.P., Feng Y., Sun Q., Xu G.Y, & Zhang H.H. (2017). Alpha-lipoic Acid suppresses P2X receptor activities and visceral hypersensitivity to colorectal distention in diabetic rats. Scientific Reports, 7, 3928.

Publication 2017

mouse anti-actin anti-rabbit peroxidase-conjugated secondary antibody anti-mouse horseradish peroxidase-conjugated secondary antibody

Actin Anti antibody Antibodies Gapdh Horseradish peroxidase Mouse Peroxidase Proteins Rabbit Rats Western blotting analysis

Corresponding Organization :

Other organizations : Soochow University, Second Affiliated Hospital of Soochow University, Suzhou University of Science and Technology, Suzhou Municipal Hospital

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Variable analysis

independent variables

Diabetes condition (diabetic vs. control)

dependent variables

Expression of P2X2 in T13-L2 DRGs
Expression of P2X3 in T13-L2 DRGs

control variables

Primary antibodies: rabbit anti-P2XRs (1:1000, Abcam, USA), rabbit anti-GAPDH (1:1000, Biotechnology Co., CHN), mouse anti-actin (1:1000; Chemicon, Temecula, CA)
Secondary antibodies: anti-rabbit peroxidase-conjugated secondary antibody (1:2000; Santa Cruz Biotechnology, Santa Cruz, CA), anti-mouse horseradish peroxidase-conjugated secondary antibody (1:4000; Chemicon)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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