Apoptosis analysis was performed as previously described (16 (link)). For flow cytometry analysis, KYSE-450 or KYSE-150 cells (1×106/well) were plated in 6-well plate and treated with DpdtC (0, 10 or 20 µM) for 20 h at 37°C. The cells were then labeled with Annexin V (20 µg/ml) and propidium iodide (PI) (50 µg/ml) (Dojindo Molecular Technologies, Inc.) at room temperature for 15 min. Apoptotic rates were analyzed using a FACSCalibur flow cytometer (BD Biosciences) and calculated using FlowJo software v.7.6.1 (Treestar, Inc.). The rate of early apoptosis was calculated by Annexin V (+) and PI (−), while the rate of late apoptosis was calculated by Annexin V (+) and PI (+).
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Apoptosis Analysis of Cancer Cells
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Corresponding Organization :
Other organizations : Xinxiang Medical University, First Affiliated Hospital of Zhengzhou University
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Variable analysis
independent variables
- DpdtC concentration (0 μM, 10 μM, 20 μM)
- Apoptotic rate (early apoptosis, late apoptosis)
- Cell line (KYSE-450, KYSE-150)
- Cell density (1×10^6 cells/well)
- Incubation time (20 h)
- Incubation temperature (37°C)
- Annexin V concentration (20 μg/ml)
- Propidium iodide concentration (50 μg/ml)
- No positive or negative controls were explicitly mentioned.
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