Measurement of ALP activity and cell viability

ALP activity was determined following the manual instructions of ALP assay kit (Abcam plc, Cambridge, UK). The color of the reaction was measured at 405 nm. The number of viable cells was determined using the Cell Titer-Blue cell viability assay according to the manufacturer’s instructions (Promega, USA) at OD 579. ALP activity was represented as fold change over control non-induced cells after normalization to the number of viable cells [40 (link)].

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Abdallah B.M., Alzahrani A.M., Abdel-Moneim A.M., Ditzel N, & Kassem M. (2019). A simple and reliable protocol for long-term culture of murine bone marrow stromal (mesenchymal) stem cells that retained their in vitro and in vivo stemness in long-term culture. Biological Procedures Online, 21, 3.

Publication 2019

ALP assay kit Cell Titer-Blue cell viability assay

Assay Cell viability Cells Promega

Corresponding Organization : King Faisal University

Other organizations : Odense University Hospital, University of Southern Denmark

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Variable analysis

independent variables

Induction conditions

dependent variables

ALP activity
Number of viable cells

control variables

Non-induced cells

controls

Positive control: non-induced cells
Negative control: not mentioned

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