Apoptosis Quantification by Flow Cytometry

Propidium iodide (PI) and Annexin V-FITC-flow cytometry assay (BD Pharmingen, CA) was used to detect the apoptosis in the cells. Cells were treated with 5-fluorouracil (5-FU) for 18–24 h and harvested in PBS. Then the cells were re-suspended in binding buffer (BD Pharmingen, CA), and stained with FITC-conjugated annexin V and PI (Kaijishengwu, China, KGA107). After staining, the cells were analysed by flow cytometry (FACS Calibar; Becton-Dickinson) using Cell Quest software [21 (link)].

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Zhang L., Zhou R., Zhang W., Yao X., Li W., Xu L., Sun X, & Zhao L. (2019). Cysteine-rich intestinal protein 1 suppresses apoptosis and chemosensitivity to 5-fluorouracil in colorectal cancer through ubiquitin-mediated Fas degradation. Journal of Experimental & Clinical Cancer Research : CR, 38, 120.

Publication 2019

Annexin V-FITC-flow cytometry assay binding buffer FACS Calibar

Annexin v fitc Apoptosis Assay Buffer Cell Flow cytometry Propidium iodide

Corresponding Organization : Southern Medical University

Other organizations : Nanfang Hospital, Guangdong General Hospital, Guangzhou Medical University Cancer Hospital, Guangzhou Medical University

Top 5 similar protocols

Variable analysis

independent variables

5-fluorouracil (5-FU)

dependent variables

Apoptosis in the cells

control variables

Cells were treated with 5-fluorouracil (5-FU) for 18–24 h and harvested in PBS
Cells were re-suspended in binding buffer (BD Pharmingen, CA), and stained with FITC-conjugated annexin V and PI (Kaijishengwu, China, KGA107)
Cells were analysed by flow cytometry (FACS Calibar; Becton-Dickinson) using Cell Quest software

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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