Genetic Manipulation of Fruit Fly Development

All fly stocks were raised on standard Bloomington medium at 25°C, and young flies (0 to 2 day old adults) were used for all experiments unless otherwise noted. The following fly stocks were used: hts⁰¹¹⁰³ (Yuan et al., 2012 (link)), nos-gal4 (Van Doren et al., 1998 (link)), UAS-α-spectrin^RNAi (TRiP.HMC04371), c587-gal4 (Decotto and Spradling, 2005 (link)), UAS-Diap1, EndoG^MB07150 (DeLuca and O'Farrell, 2012 (link)) (obtained from the Bloomington Stock Center), p53^5A-1-4 (Wichmann et al., 2006 (link); Xie and Golic, 2004 (link)), Df(2R)BSC26, Df(3R)ED6096, Df(2L)Exel7077, Df(3R)BSC699 (obtained from the Bloomington Stock Center), p53RE-GFP-nls reporter (Wylie et al., 2014 (link)) (a gift of John Abrams, University of Texas Southwestern Medical Center), Omi^Δ1, Omi^Df1 (Tain et al., 2009 (link); Yacobi-Sharon et al., 2013 (link)) (a gift of Eli Arama, Weizmann Institute of Science), mnk⁶⁰⁰⁶ (Takada et al., 2003 (link)) (a gift of William Theurkauf, University of Massachusetts Medical School), Ubi-Pavarotti-GFP (Minestrini et al., 2002 (link)) (a gift of David Glover, University of Cambridge).