Protocol detail

Indole Quantification and Analysis Workflow

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Indole concentration was measured by HPLC analysis (Hitachi Primaide, Japan)27 (link). Strain growth was characterized by optical density at 660 nm (OD₆₆₀) using UV-vis spectrophotometer (Metash UV-9000, China). The indole transformation products at the final cycle of each stage were analyzed using HPLC-MS analysis on a quadrupole ion trap instrument (Agilent HP 1100 LC/MSD, USA) equipped with an atmospheric pressure chemical ionization (APCI) source.

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Ma Q., Qu Y., Zhang X., Liu Z., Li H., Zhang Z., Wang J., Shen W, & Zhou J. (2015). Systematic investigation and microbial community profile of indole degradation processes in two aerobic activated sludge systems. Scientific Reports, 5, 17674.

Publication 2015

Primaide UV-9000

Atmospheric pressure Hplc Indole Optical Strain

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Variable analysis

independent variables

Indole concentration

dependent variables

Indole concentration measured by HPLC analysis
Strain growth characterized by optical density at 660 nm (OD660) using UV-vis spectrophotometer
Indole transformation products analyzed using HPLC-MS analysis on a quadrupole ion trap instrument equipped with an atmospheric pressure chemical ionization (APCI) source

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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