RNA was extracted from 106 cells using a Total RNA Extraction Kit (RBCBioscience, Real Genomics, New Taipei City, Taiwan cat. #YRB100) and quantified using a Nanodrop (Thermo Fisher, Waltham, MA, USA) and stored at −80 °C.
For each sample, we reverse-transcribed 2 ug of RNA using PrimeScript RT-PCR (TaKara, Kusatsu, Japan, cat. #RR047A). Real-time PCR was performed by using a QuantiNova SYBR Green PCR Kit (Qiagen, Hilden, Germany, cat. #208056) as described by Paccosi S et al. [41 (link)]. The 18S gene was used as the housekeeping gene. The primers used in this work are reported inTable 2 .
For each sample, we reverse-transcribed 2 ug of RNA using PrimeScript RT-PCR (TaKara, Kusatsu, Japan, cat. #RR047A). Real-time PCR was performed by using a QuantiNova SYBR Green PCR Kit (Qiagen, Hilden, Germany, cat. #208056) as described by Paccosi S et al. [41 (link)]. The 18S gene was used as the housekeeping gene. The primers used in this work are reported in
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