Plasmid Construction and Antibody Catalogue

pEGFP-N1-Ngb wild-type (WT) and mutants (E53Q, E118Q, K119N, H64A, H64L, Y44D)^{19 (link)} and pGenesil-1-shNgb (short hairpin Ngb)^{20 (link)} were previously constructed. pEGFP-C1-Ngb WT and truncates (Δ1–6, Δ1–18, Δ1–35, Δ1–42, Δ106–151, Δ123–151, Δ128–151), pDEST26-CV-p38, and pDEST-Flag-NV-p38 were constructed in our laboratory. EST26-CV (C-terminal 137–238 Venus), pDEST26-CV-p38, pDEST-Flag-NV (N-terminal 1–157 Venus), pDEST-Flag-NV-p38, pDEST27-GST, and pDEST27-GST-p38 were kindly provided by Professor Haian Fu (Department of Pharmacology, School of Medicine, Emory University, USA). pDEST26-CV-Ngb and pDEST-Flag-NV-Ngb were previously constructed^{18 (link)}. p-FU-p38^K53A mutant plasmid was constructed by PCR amplifying p38^K53A fragment (with stop codon) using specific primers and pDEST-Flag-NV-p38 template and then cloning into p-FU-Venus plasmids at BamH1/BsrG1sites. All plasmids were confirmed by sequencing before use. Antibodies against Ngb, Flag (Sigma, Saint Louis, MO, USA), GAP43, GFAP, NeuN, NF200, p-p38, p38 (Cell Signaling Technology, Boston, USA), β-tubulin, green fluorescent protein (GFP), Cygb, GST, His (Santa Cruz Biotechnology, Santa Cruz, USA), and Tau-1 (Merk Millipore Ltd., Darmstadt, Germany) were purchased.