Confocal Microscopy of Transfected HEK293 Cells

Transfected HEK293 cells were examined with an SP8 confocal fluorescence microscope using 63 × 1.4 NA objective, Leica (Wetzlar, Germany) as described previously^{17 (link)}. Briefly, cells were observed microscopically using an upright Olympus BX51WI equipped with 100 × 1 NA objective and images were captured using a Grasshopper3 CMOS camera (FLIR, Richmond, BC, Canada) controlled by Leica LAS X version 3.5 software (available at: https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/). All figures were created using Adobe Illustrator CS6 (Available at: https://adobe.com/products/illustrator), under an Adobe Inc., Creative Cloud Desktop 2019 shared device license to Case Western Reserve University (CWRU) that operates until 3/31/2022.

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Soler D.C., Kowatz T., Sloan A.E., McCormick T.S., Cooper K.D., Stepanyan R., Engel A, & Vahedi-Faridi A. (2021). A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line. Scientific Reports, 11, 13673.

Publication 2021

SP8 confocal fluorescence microscope 63 × 1.4 NA objective BX51WI Grasshopper3 CMOS camera Illustrator CS6

Cells Cmos Device Fluorescence microscope Hek293 cells Microscope

Corresponding Organization :

Other organizations : Case Western Reserve University, University Hospitals of Cleveland, University of Basel

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