Spiral Microfluidic Chip for Cell Separation

The microfluidic system is composed of the triplet-microchannel spiral microfluidic chip (Spiral-Slits Chip), plastic tubing, syringes and syringe pumps^{17 (link)}. A syringe pump was used to load cell suspension into Spiral chip. The syringe attached to the pump was connected to the inlet of the triplet-microchannel spiral microfluidic chip through plastic tubing³⁰. An inverted phase contrast fluorescence microscope (Nikon, Japan) equipped with a high-speed CCD camera (Nikon, Japan) was used to observe. This device was fabricated in Polydimethylsiloxane (PDMS) bonded to a glass substrate^{17 (link)}.

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, & Chen H. (2018). A Triplet Parallelizing Spiral Microfluidic Chip for Continuous Separation of Tumor Cells. Scientific Reports, 8, 4042.

Publication 2018

inverted phase contrast fluorescence microscope high-speed CCD camera

Cell Chip Device Fluorescence Phase contrast microscope Polydimethylsiloxane Syringe Triplet

Corresponding Organization : Anhui University of Technology

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Variable analysis

independent variables

Syringe pump flow rate

dependent variables

Cell behavior observed under the inverted phase contrast fluorescence microscope

control variables

Triplet-microchannel spiral microfluidic chip (Spiral-Slits Chip)
Plastic tubing
Syringes
Inverted phase contrast fluorescence microscope (Nikon, Japan) equipped with a high-speed CCD camera (Nikon, Japan)
Polydimethylsiloxane (PDMS) chip bonded to a glass substrate

controls

No positive or negative controls were explicitly mentioned in the provided information.

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