Cell Line Cultivation and Treatment Protocol

Jurkat, CEM, C8166/45, MT-2, SLB-1, and ATL-2 cells were cultured in Isocove’s modified Dulbecco medium (IMDM). Primary CD4⁺ lymphocytes, TL-Om1, and HTLV-1-immortalized lymphocyte cell lines [47 (link)] were cultured in Roswell Park Memorial Institute (RPMI) medium. HeLa and HeLa-HBZ clonal cell lines [48 (link)], CHO-LFA-1 clones [38 (link)], and HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM). All cells were supplemented with 10% FBS or 10% FetalPlex (GeminiBio) and 2 mM L-glutamine, 100 U/mL penicillin, and 50 μg/mL streptomycin. Jurkat pminLuc-viral CRE cells [38 (link)] and Jurkat-HBZ cells [38 (link)] were supplemented with 1.5 mg/mL of G418. HeLa and CHO clones were supplemented with 0.5 mg/mL of G418. Primary lymphocytes and lymphocyte cell lines were cultured with IL-2. Primary lymphocytes were activated in culture wells coated with anti-CD3 and anti-CD28 antibodies. Where indicated, cells were treated with 10 μM A485 (MedChem Express) or DMSO for 3 h.