Western Blotting for Protein Expression

Western blotting was used to detect expression levels of proteins as described previously
[18 (link),23 (link)]. We used antibodies against AQP3 (Santa Cruz Biotechnology, Santa Cruz, CA), vimentin, E-cadherin, Snail, AKT, phospho-AKT(Ser473) (Cell Signaling Technology, Beverly, MA), fibronectin (R&D systems, Minneapolis, MN), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Beyotime Institute of Biotechnology, Henan, China). Densitometric analysis of proteins was conducted and normalized against GAPDH. The PI3 kinase inhibitor LY294002, was obtained from Cell Signaling Technology (Beverly, MA).

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Chen J., Wang T., Zhou Y.C., Gao F., Zhang Z.H., Xu H., Wang S.L, & Shen L.Z. (2014). Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer. Journal of Experimental & Clinical Cancer Research : CR, 33(1), 38.

Publication 2014

vimentin E-cadherin Snail phospho-AKT(Ser473) fibronectin glyceraldehyde-3-phosphate dehydrogenase (GAPDH) LY294002

Antibodies Densitometric E cadherin Fibronectin Glyceraldehyde 3 phosphate dehydrogenase Ly294002 Pi3 kinase Proteins Snail Vimentin

Corresponding Organization :

Other organizations : Nanjing Medical University

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Variable analysis

independent variables

PI3 kinase inhibitor LY294002

dependent variables

Expression levels of proteins: AQP3, vimentin, E-cadherin, Snail, AKT, phospho-AKT(Ser473), fibronectin

control variables

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (used for normalization)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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