Histological Analysis of Mandibular Development

Hematoxylin and Eosin (H&E) staining was performed using standard protocols. For calcium deposit analysis, 7 µm transverse sections of HH39 mandibles were used with the Von Kossa Stain Kit (Calcium Stain) (Abcam, ab150687), following the manufacturer’s instructions. TRAP staining was performed on 8 μm-thick transverse sections of decalcified HH39 mandibles using an Acid Phosphatase Leukocyte (TRAP) Kit (Sigma-Aldrich, 387A) following the manufacturer's protocol. Automated cyst quantification was performed with the software CystAnalyser, with the settings for kidney cysts (Cordido et al., 2020 (link)).

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Bonatto Paese C.L., Chang C.F., Kristeková D, & Brugmann S.A. (2022). Pharmacological intervention of the FGF–PTH axis as a potential therapeutic for craniofacial ciliopathies. Disease Models & Mechanisms, 15(8), dmm049611.

Publication 2022

Von Kossa Stain Kit

Acid phosphatase Calcium Calcium deposit Cyst Cysts kidney Eosin Hematoxylin Leukocyte Mandibles Stain

Corresponding Organization :

Other organizations : University of Cincinnati Medical Center, Cincinnati Children's Hospital Medical Center, Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Masaryk University

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Variable analysis

independent variables

Hematoxylin and Eosin (H&E) staining
Von Kossa Stain Kit (Calcium Stain)
Acid Phosphatase Leukocyte (TRAP) Kit

dependent variables

Calcium deposit analysis
TRAP staining
Automated cyst quantification

control variables

7 µm transverse sections of HH39 mandibles
8 μm-thick transverse sections of decalcified HH39 mandibles
Settings for kidney cysts in CystAnalyser

positive controls

Not explicitly mentioned.

negative controls

Not explicitly mentioned.

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