The RIP kit (RIP-12RXN, Sigma-Aldrich) was used to detect the binding of miR-137, AKR1B10, and 1700020I14Rik to AGO2 protein according to the instructions [39 (link)]. The antibodies used for RIP are: AGO2 (1:100, ab32381, Abcam), and IgG (1:100, ab200699, Abcam, NC).
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