NEIL3 Knockdown and Overexpression Assay

RNA interference (siRNA) oligonucleotides targeting NEIL3 and negative control siRNAs were purchased from GenePharma (Shanghai, China). The siRNA sequences are listed in Supplementary Figure S1A. Transient siRNA transfection was carried out as described in a previous study^{24 (link)}. The NEIL3 sequence was cloned into the pLV-CMV-MCS-EF1-ZsGreen1-T2A-puro vector (Fenghui Biotechnology, Hunan, China) to construct the overexpression plasmid (Supplementary Figure S1B). Acquisition of lentivirus, packaging of lentivirus, and screening of stable cells were carried out as described in our previous study^{24 (link)}.

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Wang Q., Li Z., Yang J., Peng S., Zhou Q., Yao K., Cai W., Xie Z., Qin F., Li H., Chen X., Li K, & Huang H. (2021). Loss of NEIL3 activates radiotherapy resistance in the progression of prostate cancer. Cancer Biology & Medicine, 19(8), 1193-1210.

Publication 2021

siRNA) oligonucleotides targeting

Cells Lentivirus Neil3 Oligonucleotides Plasmid Rna interference Sirna Transfection Transient Vector

Corresponding Organization :

Other organizations : Sun Yat-sen Memorial Hospital, Sun Yat-sen University, University of Virginia, Sun Yat-sen University Cancer Center, Massachusetts General Hospital, Harvard University, Guangzhou Medical University

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Variable analysis

independent variables

SiRNA targeting NEIL3
Negative control siRNAs
NEIL3 overexpression plasmid

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Negative control siRNAs

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