Trizol was used
for the extraction of the RNA by homogenizing the brain tissue using
a polytron (VWR) device. A reverse transcription kit was used to transcribe
the RNA samples into cDNA. Real-time PCR was used under the following
conditions: 40 cycles at 95 °C for 5 min. Primer sequences are
given inTable 4 . Different
markers of mRNA expression were analyzed by real-time PCR GAPDH as
a reference using green qPCR master mix plus for assay. The PCR products’
mRNA expressions were quantified.67 (link)
for the extraction of the RNA by homogenizing the brain tissue using
a polytron (VWR) device. A reverse transcription kit was used to transcribe
the RNA samples into cDNA. Real-time PCR was used under the following
conditions: 40 cycles at 95 °C for 5 min. Primer sequences are
given in
markers of mRNA expression were analyzed by real-time PCR GAPDH as
a reference using green qPCR master mix plus for assay. The PCR products’
mRNA expressions were quantified.67 (link)
