For measuring lifespan, 3 cohorts (replicates) of approximately 80 mated male and 80 mated female flies of each genotype (n=240) were housed separately in 8 oz round bottom polypropylene bottles (Genesee Scientific, San Diego, CA, USA) inverted over 60 mm Falcon Primaria tissue culture dishes (Becton Dickinson Labware, Franklin Lakes, NJ, USA) containing 15 mL of diet. Diet dishes were replaced on alternate days without anesthesia (CO2) after tapping flies to the bottom of the bottle. Mortality was recorded daily and mortality calculations and Gompertz-Makeham maximum likelihood estimates done using WinModest (v.1.0.2) as described previously (Krishnan et al., 2009 (link)).
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Measuring Lifespan in Fruit Flies
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Corresponding Organization : Mississippi State University
Other organizations : Czech Academy of Sciences, Biology Centre, Institute of Entomology, Mayo Clinic in Arizona, University of Alabama
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Variable analysis
independent variables
- Genotype
- Lifespan
- Housing conditions (8 oz round bottom polypropylene bottles inverted over 60 mm Falcon Primaria tissue culture dishes containing 15 mL of diet)
- Diet (replaced on alternate days without anesthesia (CO2))
- Sex (mated male and mated female flies)
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