Comprehensive Western Blot Analysis

Western blotting was performed, as previously described [20 (link)]. Western blot analysis was performed using primary antibodies against phosphorylated hormone-sensitive lipase (p-HSL Ser660, rabbit, Cell Signaling), hormone-sensitive lipase (HSL, rabbit, Cell Signaling), phosphorylated perilipin1 (p-PLIN1 Ser522, mouse, VALA science), perilipin1 (PLIN1, rabbit, Santacruz), beta-actin (mouse, Santacruz), phosphor-(Ser/Thr) protein kinase A substrates (p-PKA substrates, rabbit, Cell Signaling), sirtuin 1 (SIRT1, rabbit, Cell Signaling), Estrogen Receptor Alpha Antibody A300-498A (Estrogen receptor alpha (ERα), rabbit, Bethyl laboratories INC.), adipose triglyceride lipase (ATGL, rabbit, Cell Signaling), phosphor- cAMP-response element binding protein (p-CREB ser133, rabbit, Cell Signaling), cAMP-response element binding protein (CREB, rabbit, Cell Signaling), uncoupling protein 1 (UCP1, rabbit, Alpha Diagnostic International), total oxphos rodent WB antibody cocktail (mouse, Abcam: ATP synthase subunit alpha, mitochondrial (ATP5A), succinate dehydrogenase [ubiquinone] iron–sulfur subunit, mitochondrial (SDHB), NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (NDUFB8), cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2)), and α/β tubulin (rabbit, Cell Signaling). Blots were visualized with Super Signal West Dura Substrate (Pierce, Invitrogen).

Free full text: Click here

Kim M., Im S., Cho Y.K., Choi C., Son Y., Kwon D., Jung Y.S, & Lee Y.H. (2020). Anti-Obesity Effects of Soybean Embryo Extract and Enzymatically-Modified Isoquercitrin. Biomolecules, 10(10), 1394.

Publication 2020

p-HSL Ser660 PLIN1 p-PKA substrates SIRT1 p-CREB ser133 total oxphos rodent WB antibody cocktail

Adipose Antibodies Antibody Antibody cocktail Beta actin Camp response element binding protein Cytochrome b Diagnostic Dura Hormone sensitive lipase Iron Mitochondrial Mouse Nadh dehydrogenase Phosphor Protein kinase a Rabbit Rodent Sdhb Sirt1 Subunit Succinate dehydrogenase Sulfur Synthase Triglyceride lipase Tubulin Ubiquinone Ubiquinone 8 Ucp1 Uncoupling protein 1 Western blot analysis

Corresponding Organization : Seoul National University

Other organizations : Pusan National University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of phosphorylated hormone-sensitive lipase (p-HSL Ser660)
Levels of hormone-sensitive lipase (HSL)
Levels of phosphorylated perilipin1 (p-PLIN1 Ser522)
Levels of perilipin1 (PLIN1)
Levels of beta-actin
Levels of phosphor-(Ser/Thr) protein kinase A substrates (p-PKA substrates)
Levels of sirtuin 1 (SIRT1)
Levels of Estrogen Receptor Alpha (ERα)
Levels of adipose triglyceride lipase (ATGL)
Levels of phosphor- cAMP-response element binding protein (p-CREB ser133)
Levels of cAMP-response element binding protein (CREB)
Levels of uncoupling protein 1 (UCP1)
Levels of ATP synthase subunit alpha (ATP5A), succinate dehydrogenase [ubiquinone] iron–sulfur subunit (SDHB), NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 (NDUFB8), and cytochrome b-c1 complex subunit 2 (UQCRC2)

control variables

None explicitly mentioned

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!