In immunized mice, sera were obtained at 21and 56 days after immunization. Plate-bound NP(7)-BSA and NP(25)-BSA (10 μg/ml) was used to measure antigen-specific antibodies. Class-switched serum immunoglobulin levels were detected using SBA Clonotyping System HRP kit (Southern Biotech). Antibody titers are given as -log2(dilution) x 40. Positive values were defined as those 3 s.d above mean values of the negative controls6 (link).
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