RNA extraction, complementary DNA synthesis, quantitative real-time PCR (qPCR) reactions were performed as previously described [13 (link),37 (link)]. The amplification mix was prepared using Roche LightCycler FastStart DNA MasterPLUS SYBR Green I kit following manufacturer’s instructions and real-time PCR was performed using LightCycler instrument. Oligonucleotide sequence of primers used for RealTime qPCR were reported in Table 1. GAPDH was used as internal reference and co-amplified with target samples using identical qPCR conditions. Samples were run in triplicate and mRNA expression was generated for each sample.
Other organizations :
University of Padua, Istituto Oncologico Veneto, Istituti di Ricovero e Cura a Carattere Scientifico, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, CIC bioGUNE, Polytechnic University of Turin, Candiolo Cancer Institute, Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
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