Protein and Gene Expression Analysis

An RT-qPCR and Western blot analysis were performed as reported previously [8 (link),10 (link)]. mRNA and protein samples were prepared 48 h after seeding, adenovirus transduction, or irradiation. The primers used for the RT-qPCR are summarized in Table S1. β-Actin was used as an internal control for calculating the relative mRNA expression levels. Antibodies against 75-kDa glucose-regulated protein (GRP75; sc-133137), extracellular signal-regulated kinases (ERK) 1/2 (sc-514302), GABARAP (sc-377300), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; sc-365062), parkin (sc-32282), PINK1 (sc-518052), SOD1 (sc-101523), and SOD2 (sc-133134) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

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Tsuchiya H., Shinonaga R., Sakaguchi H., Kitagawa Y., Yoshida K, & Shiota G. (2022). NEAT1 Confers Radioresistance to Hepatocellular Carcinoma Cells by Inducing PINK1/Parkin-Mediated Mitophagy. International Journal of Molecular Sciences, 23(22), 14397.

Publication 2022

sc-133137 sc-514302 SOD2 (sc-133134)

Actin Adenovirus Antibodies Extracellular signal regulated kinases 1 Gapdh Glucose Glyceraldehyde 3 phosphate dehydrogenase Grp75 Irradiation Mrna Parkin Primers Protein Sod2 Western blot analysis

Corresponding Organization : Tottori University

Other organizations : Tottori University Hospital

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Variable analysis

independent variables

Seeding
Adenovirus transduction
Irradiation

dependent variables

MRNA expression levels
Protein expression levels

control variables

β-Actin as internal control for RT-qPCR

controls

Positive controls: Not specified
Negative controls: Not specified

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