EY staining was performed as described (Heustis et al., 2012 (link)). Briefly, synchronized wildtype adult worms were washed and incubated with 0.15 mg/mL from a 5 mg/mL stock (dissolved in 70% ethanol; Eosin Y; Sigma-Aldrich, E4009) in 500 µL of liquid NGM for 3 hr in the dark. Worms are then prepped for microscopic analysis as described above. Note that Eosin Y stock should be stored at –20°C and before its use it should be incubated at 55°C for ~2 min and vigorously vortexed to ensure its solvation.
Kamal M., Tokmakjian L., Knox J., Mastrangelo P., Ji J., Cai H., Wojciechowski J.W., Hughes M.P., Takács K., Chu X., Pei J., Grolmusz V., Kotulska M., Forman-Kay J.D, & Roy P.J. (2022). A spatiotemporal reconstruction of the C. elegans pharyngeal cuticle reveals a structure rich in phase-separating proteins. eLife, 11, e79396.
Other organizations :
University of Toronto, Hospital for Sick Children, Wrocław University of Science and Technology, St. Jude Children's Research Hospital, Institute of Mathematics and Informatics, Czech Academy of Sciences, Institute of Mathematics, Peking University, Tsinghua University
Microscopic analysis of synchronized wildtype adult worms
control variables
Synchronized wildtype adult worms
Incubation time (3 hr)
Incubation in the dark
Liquid NGM as the staining medium
Eosin Y stock stored at -20°C and incubated at 55°C for ~2 min before use
controls
No explicit positive or negative controls are mentioned.
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