Hippurate, 3-hydroxyphenyl-acetic acid, and 2,4,6-trihydroxybenzoic acid monohydrate treated NK cells (2x105 cells per dose, duplicate) were isolated to determine Smad4 gene expression, Total mRNA was reverse-transcribed using iScript RT Supermix (Bio-Rad, Hercules, CA, USA). PCR was performed as described previously (40 (link)–42 (link)) with iTaq Univer SYBR Green Supermix (Bio-Rad). Human Smad4 primers were purchased from Integrated DNA Technologies (IDT), Inc. (Coralville, IA, USA). Relative expression of a gene in cells was determined by comparing the threshold cycle (Ct) of the gene against the Ct of a housekeeping gene, Gapdh.
Huang Y.W., Lin C.W., Pan P., Shan T., Echeveste C.E., Mo Y.Y., Wang H.T., Aldakkak M., Tsai S., Oshima K., Yearsley M., Xiao J., Cao H., Sun C., Du M., Bai W., Yu J, & Wang L.S. (2020). Black Raspberries Suppress Colorectal Cancer by Enhancing Smad4 Expression in Colonic Epithelium and Natural Killer Cells. Frontiers in Immunology, 11, 570683.
3 hydroxyphenyl acetic acid AcidCells Expression gene Gapdh Gene Hippurate Housekeeping gene Human Mrna Nk cells Primers Smad4 Sybr green
Corresponding Organization : City Of Hope National Medical Center
Other organizations :
Johns Hopkins University, The Ohio State University, University of Macau, ZheJiang Academy of Agricultural Sciences, Zhejiang University, Dalian Polytechnic University, Jinan University
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