Whole-mount in situ hybridization was performed as described previously (14 (link)). Brains dissected from juvenile and mature wild-type Canton-S males were used for these experiments. smFISH was performed following the published protocol (63 (link)), with the exception that we omitted (i) the overnight incubation in 100% ethanol and (ii) the bleaching steps. Fluorescence-tagged TASK7 (Quasar 570) and Dsx (Quasar 670) probes were designed and manufactured using a commercial source (LGC Biosearch Technologies). Confocal sections were acquired using an Olympus Fluoview 1000 microscope at 3-μm intervals.
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Whole-mount in situ hybridization of Drosophila brains
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Corresponding Organization : Boston Children's Hospital
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Variable analysis
independent variables
- Developmental stage (juvenile vs. mature)
- Expression pattern of TASK7 and Dsx genes
- Strain (Canton-S wild-type males)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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