Genomic Profiling of Lung Nodules

Fresh tissues were obtained from each nodule and healthy lung tissue 5 cm from each lesion. Healthy tissues were pathologically confirmed postoperatively. Genomic DNA was extracted from fresh tissues using the QIAamp DNA Tissue Kit (Qiagen, Germany). All the samples were subjected to wide panel-genomic sequencing (pan-cancer 1021-gene panel, Geneplus Technology Inc.) at the coverage depth of 1800 × . DNA from peripheral blood mononuclear cells (PBMCs) of the same patients served as a germline DNA reference. The detection and data analysis were performed as described previously [45 (link), 46 (link)].

Free full text: Click here

Yu F., Huang X., Zhou D., Zhao Z., Wu F., Qian B., Wang Q., Chen J., Liang Q., Jiang Y., Ding Q., He Q., Tang J., Wang X., Liu W, & Chen C. (2023). Genetic, DNA methylation, and immune profile discrepancies between early-stage single primary lung cancer and synchronous multiple primary lung cancer. Clinical Epigenetics, 15, 4.

Publication 2023

QIAamp DNA Tissue Kit

Cancer gene Genomic Germline Lung Patients Peripheral blood mononuclear cells Tissue

Corresponding Organization :

Other organizations : Central South University, Second Xiangya Hospital of Central South University

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Tissue type (nodule vs. healthy lung tissue)

dependent variables

Mutational profiles detected via wide panel-genomic sequencing

control variables

DNA from peripheral blood mononuclear cells (PBMCs) of the same patients served as a germline DNA reference

controls

Positive control: Healthy lung tissue samples
Negative control: Germline DNA from PBMCs

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!