Regarding rabbit polyclonal primary antibodies, anti-pan-fimbrin was a generous gift from Paul Matsudaira (National University of Singapore),39 (link) and anti-human Tmod1 was prepared in our laboratory.30 (link) With respect to mouse monoclonal primary antibodies, anti-α-actinin (nonsarcomeric, Actn1) was from Sigma-Aldrich Corp. (A5044; St. Louis, MO, USA), anti-Arp3 was from BD Biosciences (612134; San Jose, CA, USA), anti-ezrin from Sigma-Aldrich Corp. (E8897), and anti-β2-spectrin from BD Biosciences (612563). Rat monoclonal primary antibody anti-N-cadherin was a generous gift from Dietmar Vestweber (Max-Planck-Institute for Molecular Biomedicine).40 (link) Secondary antibodies were Alexa-488–conjugated goat anti-rabbit (A11008; Thermo Fisher Scientific, Grand Island, NY, USA), Alexa-488–conjugated goat anti-mouse (115-545-166, minimal cross-reaction; Jackson ImmunoResearch, West Grove, PA, USA), Alexa-647–conjugated goat anti-rat (112-605-167, minimal cross-reaction; Jackson ImmunoResearch), and Alexa-647–conjugated goat anti-mouse IgG (A21236; Thermo Fisher Scientific). Rhodamine-phalloidin (R415, Thermo Fisher Scientific) was used to stain F-actin, and Hoechst 33258 (B2883; Sigma-Aldrich Corp.) stained nuclei.