Malaria Antibody Quantification using MAP

IgG to malaria antigens was measured using MAP assay as described before [37 (link)]. Modification includes the use of magnetic plate separator instead of vacuum manifold (Luminex, Austin, Texas, Cat# CN-0269-01) and a MAGPIX reader instead of Luminex 100 instrument (EMD Millipore, Billerica, MA). The results were expressed as median fluorescence intensity (MFI). Negative and positive controls were included on each plate consisting of (a) plasma pool of 3 North Americans who had never travelled to malaria endemic areas as negative control and (b) pool of plasma from 7 Cameroonian multigravidae with high Ab levels to FV2 as positive control.