The methods for oral biofilm growth and challenge conditions were published before [14 (link), 15 (link)]. Briefly, 15 μL of CCS were added to 485 μL of CFS per well in 24-well plates and grown for 24 h or 48 h at 37 °C in a humidified Forma Series II Incubator (Thermo-Fisher Scientific, USA). CFS media was changed every 24 h.
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Oral Biofilm Growth Methodology
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Biofilm
Corresponding Organization :
Other organizations : University of California, San Francisco, University of California, Los Angeles
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Variable analysis
independent variables
- Duration of biofilm growth (24 h or 48 h)
- Biofilm growth
- Volume of CCS and CFS media per well (15 μL and 485 μL, respectively)
- Temperature (37 °C)
- Humidity (humidified Forma Series II Incubator)
- Frequency of CFS media change (every 24 h)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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