Effect of Bilirubin and Pamidronate on SHED Bone Formation

SHED were pretreated with 0 μM bilirubin, 50 μM bilirubin (Merck), and 50 μM bilirubin plus 10 μM pamidronate (Tokyo Chemical Industry) for 7 days. The cells (4 × 10⁶) were mixed with hydroxyapatite tricalcium phosphate (40 mg, Zimmer Inc., Warsaw, IN) and were subcutaneously transplanted into 8- to 10-week-old female Balb/c nude/nude (Japan CLEA, Shizuoka, Japan) as described previously [2 (link)]. The implants were harvested 4 weeks after the transplantation. Frozen sections were treated for hematoxylin and eosin staining or immunofluorescent microscopy. The mineralized tissue area is represented as a percentage of the total area as described previously [2 (link)].

Free full text: Click here

Yamaza H., Sonoda S., Nonaka K., Kukita T, & Yamaza T. (2018). Pamidronate decreases bilirubin-impaired cell death and improves dentinogenic dysfunction of stem cells from human deciduous teeth. Stem Cell Research & Therapy, 9, 303.

Publication 2018

bilirubin pamidronate

Bilirubin Cells Eosin Female Frozen sections Hematoxylin Hydroxyapatite Immunofluorescent microscopy Nude Pamidronate Tissue Transplantation Tricalcium phosphate

Corresponding Organization :

Other organizations : Kyushu University

Top 5 similar protocols

Variable analysis

independent variables

0 μM bilirubin
50 μM bilirubin (Merck)
50 μM bilirubin plus 10 μM pamidronate (Tokyo Chemical Industry)

dependent variables

Mineralized tissue area represented as a percentage of the total area

control variables

Cell density (4 × 10^6 cells)
Hydroxyapatite tricalcium phosphate (40 mg, Zimmer Inc., Warsaw, IN)
Transplantation site (subcutaneous into 8- to 10-week-old female Balb/c nude/nude mice, Japan CLEA, Shizuoka, Japan)
Transplant duration (4 weeks)

controls

Positive control: Not explicitly mentioned
Negative control: 0 μM bilirubin

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!