Protocol detail

Comprehensive Cancer Panel Sequencing

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DNA samples from #3cHCC-CCA and #3N were subjected to amplicon sequencing using Ion AmpliSeq Comprehensive Cancer Panel (Thermo Fisher Scientific, Inc.), as described previously (13 (link)). Candidates for cancer (#3cHCC-CCA)-specific single-nucleotide variants (SNVs) were identified by Tumor-Normal Pair Analysis version 5.2 of Ion Reporter (https://ionreporter.thermofisher.com/ir/).

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Ohni S., Yamaguchi H., Hirotani Y., Nakanishi Y., Midorikawa Y., Sugitani M., Naruse H., Nakayama T., Makishima M, & Esumi M. (2021). Direct molecular evidence for both multicentric and monoclonal carcinogenesis followed by transdifferentiation from hepatocellular carcinoma to cholangiocarcinoma in a case of metachronous liver cancer. Oncology Letters, 23(1), 22.

Publication 2021

Ion AmpliSeq Comprehensive Cancer Panel

Cancer Nucleotide Tumor

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Variable analysis

independent variables

DNA samples from #3cHCC-CCA and #3N

dependent variables

Candidates for cancer (#3cHCC-CCA)-specific single-nucleotide variants (SNVs)

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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