Growth Inhibition Assays and Combination Studies

Seventy-two hour growth inhibition assays and GI₅₀ concentration determination were performed as previously described [38 (link)]. For combination studies, cells were treated for 72 hours with RG7388 and conventional chemotherapies alone and in combination simultaneously at constant 1:1 ratios of 0.25×, 0.5×, 1×, 2× and 4×, or 0.125×, 0.25×, 0.5×, 1× and 2×, their respective GI₅₀ concentrations, depending on the drug solubility. Median-effect analysis and Combination Index (CI) values were determined using CalcuSyn v2 (Biosoft, Cambridge, UK). Experiments were at least n=3.