Plasmid Acquisition and Modification Protocols

The DNA plasmid of pmCherry-C1 and pEGFP-actin was purchased from Clontech Laboratories Inc., Mountain View, CA, USA. The mCherry-PMCA4b plasmid was generated as described previously [31 (link)]. The trafficking mutant pEGFP-PMCA4b-L^1167-1169A construct was prepared previously [31 (link)]. The SB-CAG-GFP-PMCA4b-CAG-Puromycin and SB-CAG-GFP-PMCA4b-LA-Puromycin constructs were generated, as described [26 (link)]. The non-functional mutant mCherry-PMCA4b-DE was created by introducing the D672E point mutation into the mCherry-PMCA4b and GFP-PMCA4b plasmids using QuikChange II Site-Directed Mutagenesis Kit (Stratagene) as described previously [32 (link)]. pCAGGS-GCaMP2-actin was a gift from Karel Svoboda (Addgene plasmid # 18928; http://n2t.net/addgene:18928; Accessed date: January 2021; RRID: Addgene_18928) [33 (link)]. The Cofilin-pmCherryC1 was a gift from Christien Merrifield (Addgene plasmid # 27687; http://n2t.net/addgene:27687; Accessed date: January 2021; RRID: Addgene_27687) [34 (link)] and CMV-R-GECO1 was a gift from Robert Campbell (Addgene plasmid # 32444; http://n2t.net/addgene:32444; Accessed date: January 2021; RRID: Addgene_32444) [35 (link)].