Conditional Knockout Mouse Lines for Cytoskeletal Proteins

All animal use was approved by the Duke Institutional Animal Care and Use Committee on 4/1/22 under approval number A060-22-03. The following mouse lines were used: Myh9^lox/lox [47 (link)] (Gift, Robert Adelstein), Myh10^lox/lox [51 (link)] (Gift, Robert Adelstein), Emx1-Cre (005628) [48 (link)] (Jackson Laboratory), Nestin-EGFP [46 (link)] (Gift, Qiang Lu), Myh14-GFP [40 (link)] (Gift, Terry Lechler). Proteomics and validation were performed on wild-type C57BL/6J mice (Jackson Laboratory). Background: Myh9^lox/lox and Myh10^lox/lox mixed C57BL/6J; SV129, Emx1-Cre and Nestin-EGFP C57BL/6J. Embryonic stage E0.5 defined as the morning the plug was identified. Control mice for Myh9 and Myh10 experiments were Myh9^lox/lox; Emx1- Cre^+/+ and Myh10^lox/lox; Emx1-Cre^+/+, respectively.

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D’Arcy B.R., Lennox A.L., Manso Musso C., Bracher A., Escobar-Tomlienovich C., Perez-Sanchez S, & Silver D.L. (2023). Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization. PLOS Biology, 21(2), e3001926.

Publication 2023

C57BL/6J mice

Animal C57bl mice Embryonic Institutional animal care and use committee Mice Nestin

Corresponding Organization : Duke University

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Variable analysis

independent variables

Genotype of mice used: Myh9lox/lox, Myh10lox/lox, Emx1-Cre, Nestin-EGFP, Myh14-GFP

dependent variables

Not explicitly mentioned

control variables

Background of mouse lines: Myh9lox/lox and Myh10lox/lox (mixed C57BL/6J; SV129), Emx1-Cre and Nestin-EGFP (C57BL/6J)
Embryonic stage E0.5 defined as the morning the plug was identified
Control mice for Myh9 and Myh10 experiments: Myh9lox/lox; Emx1-Cre+/+ and Myh10lox/lox; Emx1-Cre+/+, respectively
Proteomics and validation performed on wild-type C57BL/6J mice

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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