Cell Cycle and Apoptosis Analysis

For cell-cycle analysis, cells were harvested, fixed, incorporated with bromodeoxyuridine (BrdU), and stained with 7-aminoactinomycin D (BrdU Flow kit; BD Biosciences). The DNA content was analyzed using a cytofluorimeter, a fluorescence-activated cell sorter (FACScan; Becton Dickinson), and the ModFit software program (Verity Software House) (20 (link)). Terminal deoxynucleotidyl transferase dUTP nick end labeling staining (APO-BrdU kit; BD Biosciences) was performed to measure apoptosis, and bromodeoxyuridine incorporation was quantitated using fluorescence-activated cell sorting (BrdU Flow kits; BD Biosciences) according to the manufacturer's protocols.

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Ferrarotto R., Goonatilake R., Yoo S.Y., Tong P., Giri U., Peng S., Minna J., Girard L., Wang Y., Wang L., Li L., Diao L., Peng D.H., Gibbons D.L., Glisson B.S., Heymach J.V., Wang J., Byers L.A, & Johnson F.M. (2015). Epithelial-Mesenchymal Transition Predicts Polo-Like Kinase 1 Inhibitor-Mediated Apoptosis in Non-Small Cell Lung Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(7), 1674-1686.

Publication 2015

BrdU Flow kit FACScan APO-BrdU kit

Apoptosis Bromodeoxyuridine Cell Cell cycle Deoxynucleotidyl transferase Dutp Fluorescence

Corresponding Organization : The University of Texas MD Anderson Cancer Center

Other organizations : The University of Texas Southwestern Medical Center, Zhejiang University, Shandong Provincial Hospital, Shandong University

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Variable analysis

independent variables

Bromodeoxyuridine (BrdU) incorporation
7-aminoactinomycin D (7-AAD) staining

dependent variables

DNA content
Apoptosis

control variables

Cell harvesting
Cell fixation

controls

Positive control: None specified
Negative control: None specified

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