Arabidopsis thaliana plants were grown in a mixture of vermiculite, perlite, and peat moss at a 1:1:1 ratio in an environmentally-controlled chamber with a long photoperiod (16 hr light and 8 hr dark) at 22°C. The transgenic lines and plasmids were obtained from the Arabidopsis Biological Resource Center (ABRC, Columbus, OH), and had been deposited by Dr. B.K. Nelson [8 (link)].
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