Methionine Supplementation in High-Fat Fish Diet

The basic feed (60 g/kg fat) was made according to our previous paper [32 (link), 33 (link)], named Con; and the high-fat basic feed (120 g/kg fat) was based on our study on M. Albus [36 (link)], different levels of methionine (0, 4 g/kg, 8 g/kg, and 12 g/kg) were supplemented into high-fat diet obeyed the principle of equal nitrogen referenced our study [32 (link), 33 (link)], named as, HFD+M0, HFD+M4, HFD+M8, and HFD+M12, respectively. Experimental diets and levels of nutrition is shown in Table 1.
The method of proximate analysis (moisture, crude protein, crude lipid, and ash) of experimental feed was referenced in our paper [37 (link)]. Amino acids were determined by an automatic amino acid analyzer (Agilent-1100, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) referenced by the method reported by Wiriduge et al. [38 (link)], fatty acids were analyzed by GC-MS (Agilent 7890B-5977A, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) referenced the method reported by Jin et al. [39 (link)], as shown in Tables 2 and 3.