Metabolomic Profiling of Blood Plasma in Postoperative Delirium

The analysis of metabolic profiles of CSF samples from the nested case–control postoperative delirium cohort has previously been published^{13 (link)}. In this investigation the corresponding blood plasma samples of each of the same patients were examined by an identical kit-based methodology. Quantitative metabolomic profiling was performed using the Biocrates AbsoluteIDQ p180 (BIOCRATES, Life Science AG, Innsbruck, Austria) using a Xevo TQ-MS triple-quadrupole mass spectrometer (Waters Corporation, Milford, USA) as previously described^{26 (link)}. Briefly, this comprised of two general methods: UPLC (I-Class, Waters Corporation, UK) reversed-phase (Waters ACQUITY UPLC BEH C18 2.1 × 50 mm, 1.7 μm; UK) with multiple reaction monitoring (MRM), and flow injection analysis (FIA) also operating in MRM mode. Metabolite concentrations were calculated and expressed as micromolar (µM).

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Cuperlovic-Culf M., Cunningham E.L., Teimoorinia H., Surendra A., Pan X., Bennett S.A., Jung M., McGuiness B., Passmore A.P., Beverland D, & Green B.D. (2021). Metabolomics and computational analysis of the role of monoamine oxidase activity in delirium and SARS-COV-2 infection. Scientific Reports, 11, 10629.

Publication 2021

Biocrates AbsoluteIDQ p180 Xevo TQ-MS triple-quadrupole mass spectrometer

Blood plasma Flow injection analysis Metabolic profiles Patients Postoperative delirium

Corresponding Organization :

Other organizations : National Research Council Canada, Herzberg Institute of Astrophysics, Queen's University Belfast, University of Ottawa, Musgrave Park Hospital

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Variable analysis

independent variables

Metabolomic profiling methodology (UPLC with MRM, FIA with MRM)

dependent variables

Metabolite concentrations (expressed as micromolar, μM)

control variables

Biocrates AbsoluteIDQ p180 kit used for quantitative metabolomic profiling
Xevo TQ-MS triple-quadrupole mass spectrometer used for analysis
UPLC (I-Class, Waters Corporation, UK) reversed-phase (Waters ACQUITY UPLC BEH C18 2.1 × 50 mm, 1.7 μm; UK) with multiple reaction monitoring (MRM)
Flow injection analysis (FIA) also operating in MRM mode

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