Homology Modeling of Ssa1 and DnaK

All chemicals used in this study were purchased from Sigma-Aldrich (St. Louis, MO) unless noted otherwise. Restriction enzymes were purchased from New England BioLabs (Ipswich, MA). The ¹³C labeled glucose and ¹⁵N labeled ammonium chloride were purchased from Cambridge Isotope Laboratories, Inc. (Andover, MA). Homology modeling of Ssa1 structures in ATP and ADP state were prepared using SWISS-MODEL [88 (link)] and DnaK structures (PDB: 4NJ4 and 2KHO, respectively) as templates. Structural images were prepared with the PyMOL Molecular Graphics System, Version 1.5 Schrödinger, LLC.

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Schilke B.A., Ciesielski S.J., Ziegelhoffer T., Kamiya E., Tonelli M., Lee W., Cornilescu G., Hines J.K., Markley J.L, & Craig E.A. (2017). Broadening the functionality of a J-protein/Hsp70 molecular chaperone system. PLoS Genetics, 13(10), e1007084.

Publication 2017

Restriction enzymes 13C labeled glucose 15N labeled ammonium chloride PyMOL Molecular Graphics System,

Ammonium chloride Glucose Isotope Restriction enzymes

Corresponding Organization : University of Wisconsin–Madison

Other organizations : Lafayette College

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Variable analysis

independent variables

Homology modeling of Ssa1 structures in ATP and ADP state

dependent variables

Structural images

control variables

Chemicals used in this study were purchased from Sigma-Aldrich (St. Louis, MO) unless noted otherwise
Restriction enzymes were purchased from New England BioLabs (Ipswich, MA)
The 13C labeled glucose and 15N labeled ammonium chloride were purchased from Cambridge Isotope Laboratories, Inc. (Andover, MA)
SWISS-MODEL [88 (link)] and DnaK structures (PDB: 4NJ4 and 2KHO, respectively) as templates
PyMOL Molecular Graphics System, Version 1.5 Schrödinger, LLC

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