Microdissection of Brain Regions

Microdissection of the CA1 and thalamus brain regions was performed as described previously (Majer et al., 2012 (link), 2019 (link)). Briefly, 8 μm coronal sections were prepared from frozen brains in OCT, placed on polyethylene-napthalate (PEN) membrane slides, and stained using the LCM staining kit (Ambion) following manufacturer's recommendations. The CA1 hippocampal region and thalamus region were microdissected with the Veritas LCM instrument (Arcturus).

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Slota J.A., Medina S.J., Frost K.L, & Booth S.A. (2022). Neurons and Astrocytes Elicit Brain Region Specific Transcriptional Responses to Prion Disease in the Murine CA1 and Thalamus. Frontiers in Neuroscience, 16, 918811.

Publication 2022

LCM staining kit

Brains Frozen Membrane Microdissection Polyethylene Thalamus

Corresponding Organization : Public Health Agency of Canada

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Variable analysis

independent variables

Microdissection of the CA1 and thalamus brain regions

dependent variables

Not explicitly mentioned

control variables

Preparation of 8 μm coronal sections from frozen brains in OCT
Placing the sections on polyethylene-napthalate (PEN) membrane slides
Staining the sections using the LCM staining kit (Ambion) following manufacturer's recommendations

controls

No positive or negative controls were explicitly mentioned in the input protocol

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