This assay was done as described previously [12 (link)]. Briefly, cells cultured in 6-well plates were transfected for 24 h with luciferase reporter plasmid as well as with a Renilla luciferase expression plasmid (Promega) using the Lipofectamine 2000 reagent (Life Technologies). The cells were collected 24 h after transfection, lysed and analysed for luciferase activities using a Dual-luciferase Reporter Assay system (Promega) that already contains an internal control detectable simultaneously with the luciferase reporter gene. The activity of firefly luciferase was normalized by that of the Renilla enzyme.
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